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Web Application prompting for credentials? Error 401.2

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Hi folks.

We have IIS running on a server that is currently hosting a single website (Default Web Site) and several Web Applications.  They currently use Windows Authentication and successfully authenticate.

However I now need to add another Web Application.  Having done so, I keep getting prompted (from remote machines only) for credentials.  Which don't work even when I enter them.  I have:

- Ensured the Application Pool is the same as the 'working' web applications

- Ensured 'Authentication' is set up the same (ASP.Net, Basic and Windows Authentication all enabled)

- Ensured the permissions on the web application folder is the same as the 'working' web applications

- Ensured that the settings in the web.config are the same as the 'working' web applications

- Reset IIS

In the IIS logs for the 'working' web applications, I notice that cs-username is correctly DOMAIN\User.  However in my non-working web application this is blank (or a hyphen).

Not sure what else to check!!?  Any pointers please?


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