Non-admins receive 404 error loading CGI files

Hello,

We just recently rebuilt our web server (Windows Server 2016).  It is running IIS 10 and has CGI extensions enabled.  The site uses AD authentication.  When I load a perl CGI file and authenticate with an administrator account, the file loads.  However, when a member of a different group attempts to load a CGI file, they receive a 404 error.

Windows permissions are set so that other AD groups actually have full access to the Windows folder.  I have also granted "allow" access to several AD groups under the Authorization Rules.

Any idea why this might be occurring and how to resolve?

Thanks!